Insulin tolerance test under anaesthesia to measure tissue-specific insulin-stimulated glucose disposal
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Insulin tolerance test under anaesthesia to measure tissue-specific insulin-stimulated glucose disposal. / Fazakerley, Daniel J; Fritzen, Andreas Mæchel; Nelson, Marin E; Thorius, Ida H; Cooke, Kristen C; Humphrey, Sean J; Cooney, Gregory J; James, David E.
I: Bio-protocol, Bind 9, Nr. 2, e3146, 2019.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Insulin tolerance test under anaesthesia to measure tissue-specific insulin-stimulated glucose disposal
AU - Fazakerley, Daniel J
AU - Fritzen, Andreas Mæchel
AU - Nelson, Marin E
AU - Thorius, Ida H
AU - Cooke, Kristen C
AU - Humphrey, Sean J
AU - Cooney, Gregory J
AU - James, David E
N1 - CURIS 2019 NEXS 050
PY - 2019
Y1 - 2019
N2 - Insulin resistance is a pathophysiological state defined by impaired responses to insulin and is a risk factor for several metabolic diseases, most notably type 2 diabetes. Insulin resistance occurs in insulin target tissues including liver, adipose and skeletal muscle. Methods such as insulin tolerance tests and hyperinsulinaemic-euglycaemic clamps permit assessment of insulin responses in specific tissues and allow the study of the progression and causes of insulin resistance. Here we detail a protocol for assessing insulin action in adipose and muscle tissues in anesthetized mice administered with insulin intravenously.
AB - Insulin resistance is a pathophysiological state defined by impaired responses to insulin and is a risk factor for several metabolic diseases, most notably type 2 diabetes. Insulin resistance occurs in insulin target tissues including liver, adipose and skeletal muscle. Methods such as insulin tolerance tests and hyperinsulinaemic-euglycaemic clamps permit assessment of insulin responses in specific tissues and allow the study of the progression and causes of insulin resistance. Here we detail a protocol for assessing insulin action in adipose and muscle tissues in anesthetized mice administered with insulin intravenously.
KW - Faculty of Science
KW - Insulin
KW - Adipose tissue
KW - Muscle
KW - Glucose transport
KW - Glucose uptake
KW - Insulin tolerance test
U2 - 10.21769/BioProtoc.3146
DO - 10.21769/BioProtoc.3146
M3 - Journal article
VL - 9
JO - Bio-protocol
JF - Bio-protocol
SN - 2331-8325
IS - 2
M1 - e3146
ER -
ID: 213166605