Integrated method for quantitative morphometry and oxygen transport modeling in striated muscle
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Integrated method for quantitative morphometry and oxygen transport modeling in striated muscle. / Al-Shammari, Abdullah A; Kissane, Roger W P; Holbek, Simon; Mackey, Abigail Louise; Andersen, Thomas Rostgaard; Gaffney, Eamonn A; Kjær, Michael; Egginton, Stuart.
I: Journal of Applied Physiology, Bind 126, Nr. 3, 2019, s. 544-557.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Integrated method for quantitative morphometry and oxygen transport modeling in striated muscle
AU - Al-Shammari, Abdullah A
AU - Kissane, Roger W P
AU - Holbek, Simon
AU - Mackey, Abigail Louise
AU - Andersen, Thomas Rostgaard
AU - Gaffney, Eamonn A
AU - Kjær, Michael
AU - Egginton, Stuart
N1 - CURIS 2019 NEXS 084
PY - 2019
Y1 - 2019
N2 - Identifying structural limitations in O2 transport is primarily restricted by current methods employed to characterise the nature of physiological remodelling. Inadequate resolution or breadth of available data has impaired development of routine diagnostic protocols and effective therapeutic strategies. Understanding O2 transport within striated muscle faces major challenges, most notably in quantifying how well individual fibres are supplied by the microcirculation, which has necessitated exploring tissue O2 supply using theoretical modelling of diffusive exchange. Having identified capillary domains as a suitable model for the description of local O2 supply, and requiring less computation than numerically calculating the trapping regions that are supplied by each capillary via biophysical transport models, we sought to design a high throughput method for histological analysis. We present an integrated package that identifies optimal protocols for identification of important input elements, processing of digitised images with semi-automated routines, and incorporation of these data into a mathematical modelling framework with computed output visualised as the tissue partial pressure of O2 (PO2) distribution across a biopsy sample. Worked examples are provided using muscle samples from experiments involving rats and humans.
AB - Identifying structural limitations in O2 transport is primarily restricted by current methods employed to characterise the nature of physiological remodelling. Inadequate resolution or breadth of available data has impaired development of routine diagnostic protocols and effective therapeutic strategies. Understanding O2 transport within striated muscle faces major challenges, most notably in quantifying how well individual fibres are supplied by the microcirculation, which has necessitated exploring tissue O2 supply using theoretical modelling of diffusive exchange. Having identified capillary domains as a suitable model for the description of local O2 supply, and requiring less computation than numerically calculating the trapping regions that are supplied by each capillary via biophysical transport models, we sought to design a high throughput method for histological analysis. We present an integrated package that identifies optimal protocols for identification of important input elements, processing of digitised images with semi-automated routines, and incorporation of these data into a mathematical modelling framework with computed output visualised as the tissue partial pressure of O2 (PO2) distribution across a biopsy sample. Worked examples are provided using muscle samples from experiments involving rats and humans.
KW - Faculty of Science
KW - Mathematical modeling
KW - Skeletal muscle
KW - Image analysis
KW - Fiber type
KW - Capillary supply
KW - DTect
U2 - 10.1152/japplphysiol.00170.2018
DO - 10.1152/japplphysiol.00170.2018
M3 - Journal article
C2 - 30521427
VL - 126
SP - 544
EP - 557
JO - Journal of Applied Physiology
JF - Journal of Applied Physiology
SN - 8750-7587
IS - 3
ER -
ID: 209676700